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Figure 3


Fig. 3. ppn-1 expression in the DTC and regulation by hlh-2. Eggs from ppn-1::GFP nematodes were hatched and grown on bacteria carrying an RNAi construct for hlh-2 or on bacteria with a control plasmid. After 48 hours, Nomarski and fluorescence images were captured using identical exposure settings (A). DTC locations are indicated with arrowheads. DTC paths are diagrammed at the left. (B) Equal-sized ovals were centered around the DTCs on the Nomarski images and fluorescence intensities within the ovals were measured for ten animals from each condition using IPLab software. The average fluorescence ± s.d. for control and hlh-2 RNAi experiments are shown. (C) An embryo expressing ppn-1::GFP shows expression in the DTC precursor cells Z1 and Z4 indicated by arrowheads in the fluorescence image. The gonad primordium is outlined in the Nomarski image.





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