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Fig. 9. Differential phosphorylation of p38 isoforms by N-cadherin, LPA and activated RhoA. (A) Western blot analysis of pp38 using antibodies that recognise pp38 ${alpha}$ /ß and pp38 ${gamma}$ , total p38 ${alpha}$ /ß and total p38 ${gamma}$ in C2 cells transiently transfected with pcDNA, cN390 ${Delta}$ , L63 RhoA, T19N RhoA, MKK6EE or combinations of these. (B) Western blot analysis of pp38 using antibodies that recognise pp38 ${alpha}$ /ß and pp38 ${gamma}$ , total p38 ${alpha}$ /ß and total p38 ${gamma}$ in C2 cells treated with either control extract or NcadFc ligand. (C) As B, except that C2 cells were transfected with pcDNA or p38 ${gamma}$ HA. (D) Western blot of total and activated RhoA in response to LPA treatment. (E) Western blot analysis of pp38 using antibodies that recognise pp38 ${alpha}$ /ß, total p38 ${alpha}$ /ß and HA in C2 cells transfected with pcDNA or p38 ${gamma}$ HA and treated with LPA.

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