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Figure 3


Fig. 3. ß-catenin relieves I-mfa-mediated suppression of LEF-1. (A) ß-catenin competes with LEF-1 for binding to I-mfa. HEK 293T cells were transfected with plasmids as indicated. Immunoprecipitation was performed with an anti-GluGlu antibody and analyzed with an anti-HA, anti-GluGlu or anti-Myc antibody. (B) ß-catenin relieves I-mfa inhibition of the LEF-1 reporter gene in mammalian cells. For NIH3T3 cells, transfection was performed with the LEF-1 reporter gene system and expression plasmid as indicated in the figures. Luciferase activity was determined 24 hours after transfection.





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