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Figure 2


Fig. 2. Rab8 colocalizes with Rab11, but not with Rab4 or Rab5. HT1080 cells expressing EGFP-Rab4, EGFP-Rab5 and EGFP-Rab11 (A-C; green) and endogenous Rab8 (red) were analyzed by confocal microscopy (merge pictures). Endogenous Rab8 (D,G) in HeLa cells expressing EGFP-Rab11 (E,H) colocalizes sometimes on vesicular structures (arrows). F and I are merge pictures. Arrowheads indicate Rab8-positive vacuoles, vesicles and tubules at the leading edge. A HT1080 cell expressing GFP-Rab11 (K) and myc-Rab8-Q67L (J) show a prominent protrusion with Rab8 on tubules (arrow), the plasma membrane and in ruffles (arrowhead). Colocalization is seen on vesicles (L). Bars, 20 µM (A-D,J), 5 µM (G).





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