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Figure 4


Fig. 4. Rab8 affects cholera toxin B trafficking. Hela cell were treated with control (A,C,E) or Rab8-specific (B,D,F) RNAis for 48 hours. Alexa-595 cholera toxin were bound to the cells on ice for 30 minutes, and then CTxB was allowed to progress to the Golgi apparatus for 30 minutes at 37°C. The cells were fixed and stained for anti-GM130 (C,D) and anti-Rab8 (E,F). The percentage of cells showing CTxB-staining in Golgi was calculated for the cells treated with control RNAi contra Rab8-specific RNAi (G). Values from three separate experiments are shown as the mean ±s.e.m. 60 cells were counted per category and experiment. Bars, 20 µM.





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