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Figure 5


Fig. 5. Rab8 localizes to a tubular compartment. Confocal localization of endogenous Rab8 with ß1 integrin and transferrin receptor in three different human cell lines; HeLa carcinoma (A), HT1080 fibrosarcoma (B), and Paju neuroblastoma cells (C). HeLa cells contained Rab8-specific tubular structures that were negative for the transferrin receptor (A). In the HT1080 cells, Rab8 colocalized with ß1 integrins on vacuolar structures in the leading lamella (arrow), and sometimes also in the tail region (arrowhead) (B). Paju cells showed prominent Rab8-specific tubules that were positive for ß1 integrins (C). Bars, 10 µm.





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