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Figure 1


Fig. 1. Identification and continuity of ER in the respiratory neurons. (A) EYFP-calreticulin-transfected and Mag-Fura-2-stained neuron. Fluorescence images of Mag-Fura-2 and EYFP-calreticulin were coded by red and green, respectively, and were merged with yellow pixels indicating an overlap (42% in this image, mean 44±5% in seven cells). (B) Independent decreases in lumenal Ca2+ in the soma and in the dendrite after the local application of thapsigargin (Tg, 1 µM). Shown are ratioed images of Mag-Fura-2 fluorescence (350 or 380 nm excitation) measured before (top two panels) and 2 minutes after thapsigargin (lower two panels). Changes at application sites are 2x-enlarged in the right upper-corner of each panel. The arrows indicate the positioning of the application pipette. Similar responses were measured in four other neurons. Bar, 10 µm.





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