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Fig. 2. Motility of ER vesicles. (A) Red image shows the dwell areas of ER vesicles that were obtained as maximal values in a stack of 300 images in the Mag-Fura-2-stained neurons. A green-coded image shows the first frame and indicates initial positions of the vesicles (in the overlay all green pixels are yellow). (B) Fluorescence scans in the dendrite taken along the white line in A. The red and green traces show the dwell areas and positions of the vesicles, respectively. (C) Mean profiles across the vesicles and their dwell areas obtained by averaging linescans for 17 vesicles in dendrites. (D) Trajectories of ER vesicles in one representative dendrite. The traces were repositioned for presentation to begin in one point. Note the episodes of `wiggling' (small irregular displacements by <0.2 µm) and transportation events. (E) Kymographs of ER vesicles in the `x'-t plane where `x' corresponds to the one-dimensional curvilinear path in the dendrite. The colours of traces are the same as used in presenting the trajectories in D. Horizontal episodes correspond to the `wiggling' of the vesicles and inclined displacements represent the episodes of directed transport where velocity is given by the slope of kymographs as exemplified in the last trace. (F) Instantaneous velocities of ER vesicles and their approximation by the sum of three Gaussian curves.
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