spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 4


Fig. 4. Synaptic activity, lumenal [Ca2+] and mitochondrial potential. Neurons were stained with FM 1-43 and either Mag-Fura-2 (A-C) or TMRE (D-F). The representative traces (>=5 pairs collected in >=4 neurons) were obtained from the signals measured in the overlapping spots of FM 1-43 and either Mag-Fura-2 or TMRE fluorescence. Changes in [Ca2+]ER are presented as ratios of Mag-Fura-2 signals at 350 or 380 nm. Decreases in TMRE fluorescence indicate mitochondrial depolarisations. Note that all applied stimuli, 45 mM KCl (A,D), hypoxia (B,E), 100 µM t-ACPD (C), and 1 µM kainate (F) decreased the fluorescence of FM 1-43, indicating exocytosis.





Right arrow Return to article