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Figure 8


Fig. 8. Changes in exocytosis because of communication between mitochondria and ER vesicles. Synaptic vesicles, ER and mitochondria stained with FM 1-43, Mag-Fura-2 or Rhod-2. The ROIs are shown in the insets by blue-, green- and red-coded images, respectively (Bar, 1 µm). Exocytosis was induced by local applications of high-K+ solutions as indicated by the horizontal bars and its time-course was measured as the derivative of relative FM 1-43 fluorescence, -d({Delta}F/Fo)/dt. Shown are representative experiments performed at synaptic sites that contained both ER vesicles and mitochondria in the control (A,B) and 5 minutes after addition of 5 µM nocodazole (C) and 0.1 µM taxol (D) to the bath. Note separation of the initially contacting ER vesicles and mitochondria after nocodazole, the shortening of exocytosis in the presence of nocodazole and its prolongation by taxol.





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