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Figure 2


Fig. 2. Effects of NH4Cl and monensin on acidic organelles in SCCVII cells and L-M(TK-) fibroblasts. SCC-VII cells and M6P/IGF2R-positive L-M(TK-) fibroblasts were incubated in complete culture medium in the absence (control) or presence of either 10 mM NH4Cl or 1 µM monensin for 2 hours at 37°C. The cells were then labeled with LysoSensor Yellow/Blue (which accumulates in acidic compartments; pseudocolored in green) and DAPI (which stains nuclei; pseudocolored in red) before analysis by confocal laser-scanning microscopy. Bars, 10 µm.





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