spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 1


Fig. 1. Effect of expression of siRNA against SMU1/Smu1 on colony formation. (A) Diagram of the structures of mammalian Smu1. Black boxes, WD-repeat motifs. Locations of the sequence corresponding to siRNAs are indicated as S1, S2 and S3 above the diagram. An amino acid substitution found in the last WD-repeat of Smu1 of tsTM18 is indicated as G498R. (B) A photograph of colonies of 18H-1c30 cells after transfection of plasmid DNAs encoding siRNAs. Colonies on dishes after 12 days of incubation at 34°C or 39°C with 5 µg ml-1 puromycin were stained with methylene blue. Cells survived at 34°C after transfection of S2 or S3; however, few cells grew at 39°C or at 34°C after transfection of S1. Most cells formed colonies after transfection of an EGFP plasmid (control). (C) Colony formation at 39°C relative to that at 34°C by 18H-1c30 (upper) and 18K-1c1 (lower) cells. The mean and s.d. of six independent experiments are indicated as a solid bar and line, respectively. P values were calculated by Student's t-test. Significant differences in colony formation were found between EGFP and S2 and S3.





Right arrow Return to article