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Fig. 6. RT-PCR analysis of the Aptx, Dnaja1, Smu1 and Tnc transcripts in CHO-K1 and tsTM18 cells. Amplified regions are indicated by black boxes above the photograph. The coding sequences of Aptx, Dnaja1 and Smu1 were amplified. The FN III-type repeat 5-6 region of Tnc, which is alternatively spliced in mammalian development and in cancer progression (Joester and Faissner, 1999), was analysed. There were no changes in expression of Dnaja1, Smu1 and Tnc in CHO-K1 and tsTM18 cells incubated at 39°C. There were at least three size variants of Aptx, and the shortest one appeared in both CHO-K1 and tsTM18 cells during culture at 39°C.