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Figure 1


Fig. 1. Estimate of ERK2-GFP concentration. (A) Starved cells with an high expression of ERK2-GFP exhibited strong nuclear localization (green), independently of ERK phosphorylation (red, pERK immunofluorescence). Bar, 20 µm. (B) Relationship between the concentration of ERK2-GFP and nuclear localization in starved cells. The concentration index (CI, see Materials and Methods) measures ERK2-GFP localization: a larger CI corresponds to stronger nuclear accumulation. To extend the measures over two orders of magnitude we used variable laser power according to the best imaging conditions for each cell; data were pooled together after normalization to reference imaging conditions. The concentration of ERK2-GFP was estimated by comparing the fluorescence of the cells with artificial cells loaded with known concentrations of EGFP (supplementary material Fig. S1). In this study we selected cells with expression level less than approximately 150 nM (broken line) and that had a CI<=1 when starved.





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