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Figure 4


Fig. 4. Dynamic regulation of ERK2-GFP localization. (A) Treatment of starved cells with the phosphatase-inhibitor sodium-orthovanadate caused gradual nuclear translocation. Bar, 20 µm in all panels. (B) ERK activation and consequent nuclear accumulation required continuous activation of the MEK-ERK pathway. Cells were treated initially with FGF4 and with U0126 15 minutes later (20 µM). Blockage of MEK caused the rapid loss of nuclear fluorescence. (C) Comparison of the time course of ERK2-GFP translocation in response to FGF, FGF4+U0126 and orthovanadate. Traces from A and B have been normalized from 0 to 1, with 0 indicating the CI at the time of administration of the specific compound and 1 indicating the asymptotic value of the exponential fits. The CI decline caused by U0126 (open circles) has been inverted for a better comparison with the other data. The continuous lines are exponential fits to the top 80% of the data points.





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