JCS03287 Supplementary Material

Files in this Data Supplement:

• Supplemental Table 1 - Adobe PDF

• Supplemental Table 2 - Adobe PDF

• Movie 1 -

  Movie 1. Rrm4G-containing particles shuttle bi-directional along cytoskeletal tracks in filaments of AB33rrm4G expressing Rrm4 fused to eGfp. Time-lapse video corresponds to Fig. 3A. (QuickTime format; 672×67 pixels; frame rate 501 mseconds; display rate 300 mseconds)

• Movie 2 -

  Movie 2. Mutations in RRM3 neither affect formation nor shuttling of Rrm4G-containing particles. Filaments of AB33 derivatives carrying mutations in the RNP1 region of RRM3 domain are shown (AB33rrm4G^mR3). Time-lapse video correspond to Fig. 4C (QuickTime format; 447×47 pixels; frame rate 253 mseconds; display rate 167 mseconds).

• Movie 3 -

  Movie 3. Mutations in the N-terminal RRM1 cause defects in polarity without affecting formation or shuttling of Rrm4G-containing particles. Filaments of AB33 derivatives carrying mutations in the RNP1 region of RRM1 are shown (AB33rrm4G^mR1). Time-lapse video corresponds to Fig. 4C (QuickTime format; 447×84 pixels; frame rate 253 mseconds; display rate 167 mseconds).

• Movie 4 -

  Movie 4. Benomyl inhibits the formation of shuttling Rrm4G-containing particles in filaments of AB33rrm4G. Two time-lapse videos (separated by a blank frame) corresponding to Fig. 5A were combined. The first was taken directly after addition of benomyl (20 μM) and the second after 23 minutes of incubation. (QuickTime format; 444×58 pixels; frame rate 249 mseconds; display rate 167 mseconds).

• Movie 5 -

  Movie 5. In vivo co-localisation of Rrm4R-containing particles (red) and dynamic Tub1G-decorated microtubules (green) in strain AB33rrm4R/tub1G expressing Rrm4 fused to mRfp and Tub1 fused to eGfp. Time-lapse video corresponds to Fig. 5B. (QuickTime format; 444×155 pixels; frame rate 860 mseconds; display rate 167 mseconds).

• Movie 6 -

  Movie 6. In the absence of Kin1, Rrm4G-containing particles accumulate at the poles in filaments of AB33rrm4G/kin1Δ expressing Rrm4 fused to eGfp and carrying a deletion in kin1. Time-lapse video corresponds to Fig. 6B. Note the antiparallel shuttling of particles in the centre of the filament (QuickTime format; 448×87 pixels; frame rate 251 mseconds; display rate 167 mseconds)