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Figure 1


Fig. 1. Loss of Rrm4 causes polarity defects in filaments. (A) Colonies of AB33 and derivatives 24 hours after filament induction on charcoal-containing minimal medium plates (respective rrm4 alleles labelled at the top). (B) Filament formation was elicited in strains AB33, AB33rrm4{Delta} and AB33rrm4G for 4 hours in nitrate-containing minimal medium resulting in the nar1-promoter-mediated expression of an active bW2/bE1 heterodimer. Bar, 10 µm. (C) Graph indicating the percentage of filaments with empty sections 6 hours after filament induction. More than 40 filaments were analysed each (relevant rrm4 alleles and number of transformant below). (D) Graph (labelling as in C) indicating the percentage of cells that grew bipolarly 4 hours after filament induction. (E) Graph (labelling as in C) showing the length of the living tip cells of AB33 and derivatives measured 6 hours after filament induction (error bars indicate s.d., more than ten filaments were analysed for each strain).





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