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Fig. 4. The N-terminal RRM1 and the C-terminal PABC domain are important for function. (A) Graph indicating the percentage of filaments with empty sections 4 hours after filament induction (labelling as in Fig. 1C). The mutated alleles of rrm4 in AB33 derivatives (Fig. 2B) and the number of transformant are given below. More than 40 filaments were analysed for each group. (B) Graph showing the percentage of filaments that grew bipolarly 4 hours after induction (labelling as in A). For comparison the data for rrm4 and rrm4 (Fig. 1C,D) are given as dashed lines. (C) Filaments of AB33 derivatives 4 hours after induction. Bar, 5 µm. Frames are taken from supplementary material Movies 2 and 3. To document motility of particles, overlays of two frames that are 1 second apart are shown. The first and second frames are coloured in green and red, respectively, indicating non-motile particles in yellow (top, mutations in PABC) and moving particles in green and red (right and bottom, mutations in RRM). (D) Whisker diagram (labelling as in Fig. 3B) indicating the relative fluorescence intensity of Rrm4G-containing particles in strains AB33rrm4G^mR1 and AB33rrm4G^mR3 measured at the onset or after 6 hours of filamentation. For comparison, medians from the data obtained at 0 and 6 hours of filamentation (lower and upper dashed lines, respectively) from AB33rrm4G#1 (data from Fig. 3C) are shown. More than 80 particles were analysed for each group (number of transformant is indicated).

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