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Figure 4


Fig. 4. Codistribution potential of the DP tail with the intermediate filament desmin network. Representatives of double immunofluorescence analysis of IF-free SW13 cells transiently transfected with cDNAs encoding desmin (A,C,E,G) tagged at the C-terminus with an HA epitope and deletion mutants DP-BCS2849G (B), DP-BC (D), DP-BL (F), DP-CS2849G (H) and tagged at their N-terminus with a Myc epitope. When co-expressed, both DP BCS2849G and DP-BC colocalized with desmin. In some transfected cells, disruption and collapse of the desmin network was observed (see e.g. D and E). The DP mutant containing the B subdomain and the linker region, DP-BL2194-2566, also codistributed with desmin, while the DP mutant protein containing the C subdomain and the C-terminal extremity, DP-CS2849G, did not colocalize with desmin but was present as cytoplasmic aggregates in transfected SW13 cells. Bars, 15 µm.





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