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Figure 1


Fig. 1. Hippocampal expression of ARHGEF6 and localization in postsynaptic densities. (A) Level of expression of ARHGEF6 (filled circles), PAK3 (open circles) and CaMKII (open squares) in the CA1-3 region of the hippocampus, assessed using real-time fluorescence RT-PCR. Reverse transcription was performed with 1 µg of total RNA and a dilution corresponding to 16 ng of total RNA was amplified in a SYBRGreen PCR reaction. Data are mean of triplicate samples. (B) A pyramidal neuron co-transfected with EGFP (green, left, GFP) and HA-tagged ARHGEF6 (red, right, HA-GEF) showing a diffuse cytoplasmic and a punctate dendritic distribution of HA-ARHGEF6. (C) Magnified view of a dendritic branch obtained from a neuron co-transfected with EGFP (green, left, GFP) and HA-ARHGEF6 (red, right, HA-GEF), showing the punctate distribution at the level of spines. (D) A neuron co-transfected with PSD-95-EGFP and HA-ARHGEF6 showing the colocalization of PSD-95-EGFP puncta (PSD-95, green, left) with HA-ARHGEF6 (HAGEF, red, right). (E) Colocalization of synaptophysin immunostaining (green) with HAARHGEF6 puncta (red). Bars, (B) 20 µm; (C-E) 2 µm.





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