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Figure 3


Fig. 3. Effects of SPC and TGF-ß3 on the transcriptional levels of smooth-muscle-specific markers. (A) Serum-starved hATSCs were treated with vehicles, 2 µM D-erythro-SPC or 2 ng/ml TGF-ß3 for the indicated time, and {alpha}-SMA mRNA was quantified by real time RT-PCR analysis. Data represent average values ± s.e.m. of triplicate determinants. *Significantly different from control value (P<0.05). (B) Serum-starved hATSCs were exposed to 2 µM D-erythro-SPC or 2 ng/ml TGF-ß3 for 24 hours, and the levels of {alpha}-SMA, h1-calponin, SM22{alpha} and GAPDH mRNA were determined by semi-quantitative RT-PCR using gene-specific primers. Representative data from three independent experiments are shown. (C) hATSCs were treated with vehicles, 2 µM D-erythro-SPC or 2 ng/ml TGF-ß3 for 24 hours, and the expression levels of h1-calponin and SM22{alpha} were quantified by real time RT-PCR. Data represent average values ± s.e.m. of triplicate determinants. *Significantly different from control value (P<0.05).





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