spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 9


Fig. 9. The role of SRF and myocardin in the SPC-induced differentiation of hATSCs to SMCs. Serum-starved hATSCs were treated with vehicles, 2 µM D-erythro-SPC, or 2 ng/ml TGF-ß3 for 24 hours, and SRF and myocardin mRNA levels were determined by semi-quantitative RT-PCR (A) or real time RT-PCR (B). Representative data from three independent experiments are shown in A. The data in B are shown as mean ± s.e.m. (n=3). (C) hATSCs were transfected with control siRNA or siRNAs specific for SRF or myocardin, respectively. The levels of SRF and myocardin mRNAs were determined by semi-quantitative RT-PCR. Representative data from three independent experiments are shown. (D) The siRNA-transfected hATSCs were treated with vehicle, 2 µM D-erythro-SPC, or 2 ng/ml TGF-ß3 for 4 days, and the expression levels of {alpha}-SMA and actin were determined by western blot analysis. (E) The quantity of {alpha}-SMA and actin were determined in triplicate, and the expression levels of {alpha}-SMA were normalized to total actin levels in the samples. The data are presented as a percentage of mock-treated control. *Significantly different from control value (P<0.05).





Right arrow Return to article