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Figure 4


Fig. 4. Overexpression of cdi in eye discs. Clones of cells overexpressing cdi are labeled with GFP (green). (A-C) Clone anterior to the morphogenetic furrow where cells overexpressing cdi concentrate more F-actin (red) in the cell cortex. Upper panels show a cross-section, lower panels a transverse section. (D-G) Detail of a confocal cross-section at the apical region of developing ommatidia posterior to the morphogenetic furrow stained with phalloidin (red) and anti-ß-catenin (blue). Note the F-actin accumulation in the cdi-overexpressing cells (F). Arrows point to a cdi-overexpressing ommatidial cluster and arrowhead to a wild-type cluster. Localization of ß-catenin in cdi-overexpressing cells is displaced basally (G). Left panels: cross-sections. Right panels: transverse section through a mutant ommatidial cluster adjacent to a wild-type cluster. (H-K) Apical cross-section through ommatidial clusters stained with Fasciclin III (blue) and DE-cadherin (red). Note that the DE-cadherin of the closely apposed photoreceptor membranes in wild-type cells (arrowhead) is absent in the cdi-overexpressing ommatidial cluster (arrow). However, in a transverse section containing a cdi-overexpression cluster (arrow) next to a wild-type cluster (arrowhead), DE-cadherin is found more basally and in an abnormal arrangement (right panels). Fasciclin III, used to mark the cell contour, denotes that the cdi-overexpressing cluster is not shrunk or smaller. Diagonal lines in D and H indicate the region represented in the transverse sections. Bars, 10 µm.





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