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Fig. 5. Loss of cdi function in the developing eye. (A-C) Clones of cdi^R47 (lack of GFP in A and B) show a lack of Cdi expression as assayed by anti-Cdi (red in A and C). The confocal image was taken at the level of inner photoreceptors of a late third instar larva where a transient accumulation of Cdi is found. (D-F) Mitotic clones of homozygous cdi^R47 cells (lack of GFP). All these clones were supplied with UAS-mRpL55 under GMR-Gal4 driver. Actin organization is severely altered in mutant ommatidia (arrow). Clones containing both wild-type and mutant cells appear normal (arrowhead). (G-J) Clone of cdi mutant cells (lack of GFP) counterstained with anti-ß-catenin (blue) and anti DE-cadherin (red). A single confocal cross-section (upper panels) taken at the level of the adherens junctions of the wild-type cells. Note that in this level of the mutant sector, both proteins are absent. Transverse section (lower panels) where ß-catenin and DE-cadherin are aberrantly positioned. (K-N) Clones of cdi^R47 cells (lack of GFP) complemented with the double transgene UAS-mRpL55 and UAS-cdi under the GMR-Gal4 driver and stained for phalloidin (red), and ß-catenin (blue). (O) Transverse section of another clone (lack of GFP) as in K stained with DE-cadherin (red). Top, merged; middle, GFP; bottom, DE-cadherin. (P) Eye of a fly showing cdi^R47 mutant clones (white sectors, arrow) in Minute⁺ background. (Q) Semi-thin section of a mosaic eye, showing a clone of cdi^R47 mutant ommatidia (absence of inter-ommatidial pigmentation; arrow points to mutant cells). (R-T) Clones of cdi^R47 supplied with UAS-mRpL55 and stained with anti-caspase-3. Thin lines represent the plane of transverse sectioning. Bars, 10 µm.

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