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Fig. 7. Sev RTK localization in cdi gain- and loss-of-function clones. (A-D) Upper panels show a cross-section confocal image of cdi gain-of-function clones labeled with GFP (green) and F-actin (red). Lower panels show a transverse section of the same preparation taken through the region indicated with the white line in A. Sev RTK protein (blue) localization is extended to a more basal position in the mutant ommatidia (arrowheads). (E-G) Loss-of-function clone marked by the absence of GFP. Note that in some mutant ommatidia (arrow) Sev RTK (blue) staining is lower than in the wild-type. (H) Transverse sections of a loss-of-function clone where the Sev RTK staining (blue) of the apical membrane (arrow) is reduced. Bars, 10 µm.