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Figure 6


Fig. 6. Inhibition of bipolar spindle attachments can rescue inner centromeric cohesion and protein passenger localization in the absence of cohesin. (A-D) Fluorescence images of spindle reactions treated with the Eg5 inhibitor monastrol (125 µM) either prior to spindle assembly for 30 minutes (A,B), or for 30 minutes following spindle assembly (C,D) in mock- and cohesin-depleted extracts. DNA is blue, microtubules are red and Ndc80, INCENP or aurora B are green. Bar, 10 µm.





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