Plant G protein heterotrimers require dual lipidation motifs of G and G and do not dissociate upon activation

JCS03284 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. Protoplasts co-expressing (A) CFP-AGβ1 and (B) YFP-AGG2; (C) the overlayed images of A and B. Bar, 10 μm.

• Supplemental Figure 2 -

  Fig. S2. Protoplast co-expressing (A) RFP-AGβ1 and (B) YFP-AGG2(C95S). Bar, 10 μm.

• Supplemental Figure 3 -

  Fig. S3. Subcellular location and dynamics of GPα1(Q222L)-YFP. (A) Protoplasts expressing GPα1(Q222L)-YFP. Bar, 10 μm. (B) FRAP curves of GPα1-YFP (diamonds) and GPα1(Q222L)-YFP (triangles).

• Supplemental Figure 4 -

  Fig. S4. Distribution of phase lifetimes plotted against modulation lifetimes observed for (A) AGβ1 + YFP-AGG1 (closed circles) and RFP-AGβ1 + YFP-AGG1 (open circles); (B) AGβ1 + YFP-AGG2 (closed circles) and RFP-AGβ1 + YFP-AGG2 (open circles); (C) AGβ1 + YFP-AGG2mut (closed circles) and RFP-AGβ1 + YFP-AGG2mut (open circles); (D) GPα1-YFP + AGβ1 + AGG1 (closed circles) and GPα1-YFP + RFP-AGβ1 + AGG1 (open circles) and (E) GPα1(Q222L)-YFP + AGβ1 + AGG1 (closed circles) and GPα1(Q222L)-YFP + RFP-AGβ1 + AGG1 (open circles).

• Supplemental Figure 5 -

  Fig. S5. Protoplast co-expressing (A) GPα1(G2A)-YFP and non-fluorescent AGG1 and (B) CFP-AGβ1 and non-fluorescent AGG1. Bar, 10 μm.