JCS03297 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. Intercellular junctions in the HT29-derived subclone 16E. Immunolocalisation of occludin and β-catenin. Cells grown to a high density were processed for immunofluorescence using a rabbit anti-occludin and a mouse anti-β-catenin antibody followed by Alexa Fluor 488-conjugated goat anti-rabbit and Cy3-conjugated goat anti-mouse secondary antibodies. Shown are confocal xy and xz sections. The position of the latter is indicated with arrowheads.

• Supplemental Figure 2 -

  Fig. S2. Localisation of symplekin in the HT29-derived subclone 16E. Cells were fixed and processed for immunofluorescence using mouse anti-symplekin antibodies together with a rabbit anti-occludin (A,B) or a goat anti-β-catenin antibody (C,D). Shown are confocal xy (A,C) and xz(B,D) sections. Note that symplekin co-localises with junctional occludin but not the dot-like cytoplasmic occludin staining.