spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 2


Fig. 2. Association of ZONAB with symplekin. (A) Detergent extracts of MDCK and Caco-2 cells were precipitated with anti-ZONAB immunobeads or control beads. After washing, the precipitates were analysed by immunoblotting with anti-ZONAB, anti-symplekin and anti-ZO-1 antibodies. (B) Detergent extracts of HT29-16E cells were precipitated using an anti-symplekin antibody, an anti-ZO-1 antibody, or pre-immune serum (control IP) and protein G agarose beads. After washing, the precipitates were analysed by immunoblotting with anti-symplekin and anti-ZO-1 antibodies. (C) A fraction enriched in nuclei was purified by density centrifugation, solubilised and the presence of ZONAB/symplekin complexes was assayed by co-immunoprecipitation as in A. (D) MDCK cell extracts were loaded on beads carrying either a GST-ZONAB fusion protein or GST alone. After washing, the presence of symplekin was analysed by immunoblotting. (E) Recombinant His6-tagged ZONAB was loaded onto beads with bound GST-symplekin or GST. After washing, pull down of recombinant ZONAB was tested by immunoblotting. All extract and input lanes represent 10% of total inputs.





Right arrow Return to article