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Figure 3


Fig. 3. Symplekin modulates transcriptional activity of ZONAB. MDCK cells at 90% density were transfected with the ZONAB-specific reporter constructs together with the indicated expression and RNAi plasmids. (A) Cells were transfected with increasing amounts of a symplekin expression vector. The total DNA concentration was kept constant by adding corresponding amounts of empty expression vector. Luciferases were assayed either 1 or 2 days after transfection. Because ZONAB functions as a repressor in this assay, reduced promoter activity represents increased ZONAB activity. (B,C) The luciferase constructs were transfected together with the indicated expression and RNAi plasmids and the luciferases were assayed 1 day after transfection. Analogous results were obtained when the transfections were left for 2 days. Note that normal ZONAB expression is required for symplekin to affect the promoter (C). Values are derived from representative experiments performed in triplicates. (*Values that are statistically significantly different from control transfections using a t-test.)





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