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Figure 1


Fig. 1. NO/cGMP increase sarcoglycan production by intra-arterially delivered mesoangioblasts. Mesoangioblasts (5x105) pretreated for 12 hours without (NT) or with DETA-NO (20 µM), IMN (50 µM), 8-Br-cGMP (1 mM) or DETA-NO plus ODQ (1 µM) were injected into the right femoral artery of 4-month-old {alpha}SG-null mice. Mice were sacrificed after 21 days and quadriceps (Qd), gastrocnemius (Gs) and soleus (So) muscles collected. (A) {alpha}SG mRNA expression, an index of mesoangioblast homing, was measured by real-time PCR. Values ± s.e.m. are expressed as the percentage of the total injected cells (n=3). (B,C) {alpha}SG expression, evaluated by western blot analysis (B, showing both a representative western blot image and densitometric values, n=3) and immunohistological detection (C, representative of three consistent experiments). The graph in B reports the ratio of densitometric values ± s.e.m. of {alpha}SG vs. those of glyceraldehyde-3 phosphate dehydrogenase (GAPDH) used as an internal loading control. Triple asterisks and crosses, P<0.001 vs NT and DETA-NO-treated cells, respectively. Bar in C, 400 µm.





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