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Fig. 4. NO/cGMP protect mesoangioblasts from cell death-inducing stimuli. (A,B) Mesoangioblasts were pretreated for 12 hours without (NT) or with DETA-NO (20 µM), IMN (50 µM), 8-Br-cGMP (1 mM) or DETA-NO plus ODQ (1 µM) and incubated for 24 hours in the absence (NT) or presence of TNF (100 ng/ml), H₂O₂ (1 µM) or As₂O₃ (20 µM). (A) Cell death was determined by flow cytometry measuring Annexin V staining of phosphatidylserine exposed on the outer leaflet of the plasma membrane and PI incorporation, and expressed as the percentage of that observed in controls (i.e. mesoangioblasts pretreated without drugs and incubated in the absence of apoptogens) (n=4). (B) Representative dot-plot analyses showing the results obtained in one of four consistent experiments using As₂O₃ as the cell death-triggering stimulus. (C) Protection by NO/cGMP of mesoangioblasts (target) from cell death (specific lysis) induced by incubation for 16 hours with cytotoxic T lymphocytes (effector) at the indicated effector/target ratios. Single, double and triple asterisks and crosses, P<0.05, P<0.01 and P<0.001, respectively vs. NT (asterisks) or DETA-NO-treated cells (crosses). Error bars, s.e.m.

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