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Figure 5


Fig. 5. RSL1D1 has a longer nucleolar retention time than nucleostemin. (A) Time-sequenced FRAP images of nucleostemin and RSL1D1 in the nucleolus. A circle of 1 µm in diameter within the nucleolus (arrows) was bleached. Of note, low intensity spots in the upper panels (*) existed before photobleaching. Numbers indicate the time in seconds after the bleaching event. Bars, 1 µm. (B) The FRAP recovery curves of RSL1D1 and nucleostemin depict the average of the fluorescence recovery level (y-axis; n=20) relative to the prebleached intensity (set as 1) over a 31.6-second period following photobleaching (x-axis) in seconds. Error bars represent ± standard deviations (±s.d.) and are omitted on the top and bottom side of the RSL1D1 and nucleostemin recovery curves for clarity. (C) t-test analyses of the FRAP results were conducted at 5, 10, 20 and 30 seconds after photobleaching (mean ± s.e.m.; n=20).





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