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Figure 8


Fig. 8. Overexpression of a nucleolar form of the L1-domain (nlsL1) disperses nucleostemin from the nucleolus. (A1) The intensities of nucleostemin signals in the nucleolus are diminished or disappear in many cells that express the nlsL1 mutant. (A2) High magnification of the nlsL1-expressing cell show that its remaining nucleostemin signals display a reticular pattern of distribution, similar to the nucleostemin distribution in wild-type cells. (B) In some cells, nucleostemin is scattered around the nlsL1 signals. Overexpression of the nlsL1 mutant does not affect the signal intensities or the distribution patterns of fibrillarin (C) or B23 (D). Bars, 10 µm for A1; 5 µm for A2, B, C, D.





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