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Figure 3


Fig. 3. Co-immunoprecipitation of WRN, RAD51 and ATR. (A) WRN is pulled down with RAD51 (H-92) from whole cell extracts prepared from HeLa cells treated with MMC (0.5 µg ml-1), lane 5. In a WRN pulled down with the rabbit polyclonal anti-WRN (ab17987) we co-immunoprecipitated RAD51, lanes 7 and 8. Mouse anti-WRN (BD) or polyclonal anti-WRN (ab17987) and anti-RAD51 (ab1837) were used for the WBs. (B) WRN is pulled down with ATR from whole cell extracts prepared from HeLa cells. Rabbit polyclonal anti-ATR (ab91) and anti-WRN (ab200) were used for IP, and monoclonal mouse anti-WRN (BD) and anti-ATR (ab91) were use for WB. Input represents whole cell lysate. IgG is normal rabbit IgG (SC).





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