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Figure 1


Fig. 1. Cytoskeletal organisation at MCF-7 scratch wound edges. (A) Phalloidin-stained actin cytoskeleton 30 minutes after wounding. (B) Actin cytoskeleton 2 hours after wounding. (C) SEM image of the cell surface of a wound edge cell 30 minutes after wounding displaying numerous microspikes but only a few small lamellipodia. (D-H) Immunofluorescence staining of Keratin 8 at the wound edge. (D) Low-power overview of K8 network at wounded epithelia 2 hours after wounding. (E) High-power image of K8 at the wound edge 30 minutes after wounding. (F) K8 at the wound edge 2 hours after wounding. (G) Pseudocolour gradient representation of the staining intensity form panel E. Yellow colour marks the most intense staining that is mostly localised at a cage around the nucleus. (H) Pseudocolour gradient representation of the staining intensity from panel F showing most intense keratin staining at the wound edge. (I) Percentage of cells showing the strongly stained keratin cable immediately parallel to the wound edge 30 minutes and 2 hours after the wounding. 200 cells were scored from three independent wounds. The scale bar shown in F equals to 20 µm in A, B, E-H; 50 µm in D and 200 nm in C.





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