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Figure 2


Fig. 2. Field emission scanning electron microscopy of the intermediate filament network at MCF-7 scratch wound edges. Confluent monolayers grown on silicone discs were scratch wounded. After 30 minutes or 2 hours the cells were extracted and depleted from filamentous actin. (A,B) Two representative example of the wound edge IF cytoskeleton 30 minutes after wounding. (C,D) Two examples of IF organisation at the wound edge 2 hours after the wounding. (E) High magnification of the keratin cable at the wound edge. (F) Individual chromium-coated filaments at 200,000x magnification. The scale bar corresponds to 500 nm in A-D, 175 nm in E and 55 nm in F.





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