spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 6


Fig. 6. Downregulation of periplakin and keratin 8 expression by siRNA transfections. (A) western blotting of total cell extracts from transient transfections with control or specific siRNAs. Total cell extracts were collected 72 hours after transfection and 20 µg protein aliquots were blotted with antibodies against indicated proteins. (B) Immunofluorescence staining of MCF-7 cells transfected with scrambled control siRNA (top row), Periplakin siRNA (middle row) or K8 siRNA (bottom row) showing K8 staining (red channel on the left) Periplakin staining (green channel, middle column) and merged image (on the right). (C) Control and K=8 siRNA transfected cells stained with Desmoplakin antibody (green channel, on the left) and merged with K=8 antibody staining (red channel). Scale bar is 20 µm in both B and C.





Right arrow Return to article