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Figure 8


Fig. 8. Wound closure in Keratin 8 siRNA downregulated MCF-7 epithelial sheets. (A) Phase contrast micrographs of the closure of control or K8 siRNA wounds at indicated time points. Cell layers were wounded 48 hours after transfection. Note the loss of epithelial phenotype in K8 siRNA transfected cells at the 20 hours time point. (B) Keratin (red) and desmoplakin (green) immunofluorescence of a control siRNA transfected wound. (C) Keratin (red) and desmoplakin (green) immunofluorescence of a K8 siRNA transfected wound. Arrowhead indicates a group of cells with no keratin immunofluorescence that have migrated away from the cell front to wound space. (D) Keratin (red) and Periplakin (green) immunofluorescence of K8 siRNA transfected wound. (E) Magnification of the area boxed in D. Note, how cells with low keratin expression have mostly cytoplasmic localisation of periplakin. Scale bar in B-D is 50 µm and in E 17 µm.





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