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Figure 2


Fig. 2. GST-Grp1-PH labeling on ultrathin thawed cryosections of Swiss 3T3 cells. Ultrathin cryosections of cells stimulated with 50 ng/ml PDGF were labeled with GST-Grp1-PH as described in the Materials and Methods. GST-Grp1-PH immunogold labeling, indicated by arrows, is visualised on the plasma membrane (PM) and endoplasmic reticulum (ER) of cells stimulated with PDGF for 5 minutes (A) and unstimulated cells (B). (C) GST-Grp1-PH labeling is located on a lamellipodium structure and plasma membrane (PM) of cells stimulated with PDGF for 2 minutes. Nuclear labeling in non-stimulated and stimulated (PDGF for 5 minutes) cells is shown in D and E, respectively. Arrows in F, G and H indicate gold labeling on internal vesicles of a MVB, Golgi stack and caveolae-like structures, respectively. Bars, 200 nm.





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