JCS03299 Supplementary Material

Files in this Data Supplement:

• Movie 1 -

  Movie 1. A 5 day post-fertilization control embryo was fixed and stained to visualize actin (red) and acetylated tubulin (green) in the anterior macula. Kinocilia in controls are prominent and extend well beyond the actin-rich portion of the hair cell bundle. The microtubule network is extensive within the cell body of each hair cell. Image volume was acquired using a BioRad MRC 1024 Laser Confocal Microscope system equipped with a Nikon Diophot microscope, 100× oil objective 1.4 NA and multiphoton illumination. Volumes were rendered to produce the movies using Voxx software. Width of field is 35 μm. Thickness of volume is 12 μm. Two planes from this volume were used to produce the main image in Fig. 1E. The top inset in Fig. 1E is a projection image of this movie volume.

• Movie 2 -

  Movie 2. A 5 day post-fertilization cdh2 morpholino oligonucleotide injected embryo was fixed and stained to visualize actin (red) and acetylated tubulin (green) in the anterior macula. Kinocilia in Cdh2 knockdowns are shorter than those in control embryos. Cytoplasmic microtubules are also less elaborate than in control hair cells. Image volume was acquired using a BioRad MRC 1024 Laser Confocal Microscope system equipped with a Nikon Diophot microscope, 100× oil objective 1.4 NA and multiphoton illumination. Volumes were rendered to produce the movies using Voxx software. Width of field is 35 μm. Thickness of volume is 12 μm. Two planes from this volume were used to produce the main image in Fig. 1F. The top inset in Fig. 1F is a projection image of this movie volume.