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Figure 12


Fig. 12. Angiogenesis. (A-F) von Willebrand factor staining of nacent blood vessels granulation tissue (green) with bis-benzimide nuclear stain (blue) at 7 days (A,B), 10 days (C,D) and 14 days (E,F) after wounding. In Cx43-asODN-treated wounds (B,D,F) blood vessels were more pervasive at early time points (7 days, B; 10 days, D) but considerably finer than those of controls (A,C,E) resulting in significantly reduced staining compared with controls (7 days *P=0.0019; 10 days **P=0.015). By 14 days, blood vessels had increased in size in the asODN group and were a similar size to those of controls (G). (H) Illustration of sites imaged in the granulation tissue. Zone I (A,B) zone II (C-E). e, epidermis; d, dermis. Per treatment and time point six to eight animals were used. Data are expressed as the mean ± s.e.m. Bars, 25 µm. For a high-resolution figure, please see the online version of this article.





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