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Fig. 2. Cell proliferation. (A-D) Analysis of cell proliferation at wound sites by immunohistochemical staining with the anti-BrdU monoclonal antibody in control ODN (A, day 2; C, day 7) and Cx43-asODN (B, day 2; D, day 7). Arrowhead and arrow indicate the wound margin and leading edge, respectively. (E,F) The number of BrdU-positive cells per field in the wound margin in the epidermis (E; n=5) and the nascent epidermis (F; n=5). (G,H) The number of BrdU-positive cells per 1.33 mm2 in the dermal wound edge (G; n=5) and in the forming granulation tissue (H; n=5). Counts are expressed as the mean ± s.e.m.; *P<0.05. Bars, 200 µm.