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Figure 6


Fig. 6. Protein velocity maps for 3T3 cells plated on 1 µg/ml fibronectin. (A) Velocity maps of EGFP conjugated paxillin (analysis on 121 frames, 5 seconds/frame), FAK (analysis on 200 frames, 10 seconds/frame) and talin (52 frames, 9 seconds/frame), and the corresponding mRFP-actin velocity maps. Insets are a 2x expansions of the small white box in each image. Velocity scale arrows are 1 µm/minute, spatial scale bars are 5 µm. (B) Plot of the median relative magnitude and directional correlation coefficient scores relative to actin for each protein. Error bars are relative error in the velocity measurement.





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