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First published online December 11, 2006

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G-proteins go green...

Heterotrimeric G proteins are important signal transducers for 7-transmembrane-span receptors. In mammals, receptor activation leads to exchange of GDP bound to the G protein subunit (G) for GTP. G and Gß then dissociate and activate various effectors. On p. 5087, Theodorus Gadella and co-workers provide the first evidence for a heterotrimeric G protein in a dicot plant. This, they reveal, behaves very differently from mammalian G proteins. Lipid modifications are important for the function of mammalian G proteins. To discover their role in the formation and localization of plant G protein heterotrimers, the authors used fluorescence resonance energy transfer (FRET) with fluorescence lifetime imaging microscopy (FLIM) to follow GFP-tagged Arabidopsis G-protein subunits in cowpea cells. Localization of G (GP1) and the two G subunits (AGG1 and AGG2) to the plasma membrane, they report, requires modification of two lipidation motifs in each subunit. More importantly, they show that GP1-AGß1-AGG1 heterotrimers exist at the plasma membrane but do not dissociate upon activation of GP1; instead these may toggle between two conformations on GDP/GTP exchange.

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Related articles in JCS:

Plant G protein heterotrimers require dual lipidation motifs of G and G and do not dissociate upon activation

Merel J. W. Adjobo-Hermans, Joachim Goedhart, and Theodorus W. J. Gadella, Jr
JCS 2006 119: 5087-5097. [Abstract] [Full Text]  

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Related articles in JCS Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Search for Related Content Social Bookmarking                         What's this?