JCS02761 Supplementary Material

Files in this Data Supplement:

• Movie 1a
• Movie 1b -

  Movie 1. F-actin dynamics visualized by Moesin-GFP during wild-type denticle development.  Still images are presented in Fig. 2.  (a)  Note the cell shape changes that precede apical actin accumulation in the cells of the denticle belt.  Actin accumulation begins laterally and spreads medially. (b)  Bright puncta of actin appear along the posterior margins of denticle-producing cells.  Some puncta are motile and travel along the posterior margin, and puncta sometimes merge with adjacent puncta.  Condensations then take on a more pointed shape and begin to elongate.  Frames displayed were collected every 30s.  Movie play rate is 10 frames/s.

• Movie 2 -

  Movie 2. APC2 localization visualized with APC2-GFP during wild-type denticle development.  Still images are presented in Fig. 7.  Note the similarity of APC2’s localization pattern to that of F-actin (Movies 1a/1b).  Frames displayed were collected every 45s. Movie play rate is 10 frames/s.

• Movie 3a
• Movie 3b -

  Movie 3. Fz localization visualized with Fz-GFP during wildtype denticle development.  Still images are presented in Fig. 8.  Movie 3b is an enhanced contrast version of the first 50 frames of Movie 3a. Fz-GFP initially localizes to motile puncta we hypothesize are vesicles, some of which are cytoplasmic and some associated with the cortex.  Fz-GFP then becomes more cortical, and in denticle-producing cells assumes a somewhat asymmetric distribution.  Frames were collected every 1 minute.  Movie play rate is 10 frames/s.

• Movie 4 -

  Movie 4. F-actin dynamics visualized by Moesin-GFP during denticle development in wg^IG22. Compare with Movies 1a and 1b.  Apical actin accumulation is seen at the beginning of the movie.  Note that condensations do not always form along the posterior margin, but instead form along other margins of the cells or in the middle of the apical domain.  Note also that condensations focus very rapidly.  Frames displayed were collected every 45s. Movie play rate is 10 frames/s.