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Figure 2


Fig. 2. Denticles develop from polarized actin accumulations. Embryos, anterior left or upper left, F-actin (green or single label; A-C,E-H=phalloidin; D=anti-actin) and P-Tyr (B,E-H) or Arm (D) to outline cells (red). Insets=close-ups. (A) Extended germband. (B,C) Cells of presumptive denticle belts accumulate apical actin (brackets, B inset), beginning laterally (B, bottom left). (C,D) Actin concentrates along posterior cell margins (arrows). Insets B,D are close-ups. (E) Actin condenses into foci along the posterior margin (arrows). Inset, rare error in denticle positioning in row 1 (arrowhead). (F,G) Stages in denticle elongation (arrows, arrowheads, multiple denticles/cell). (H) Dorsal hairs. Condensations form and hairs (inset) elongate at the posterior-margin (blue arrowheads) or anterior-margin (white arrowheads) in different cell rows. (I-P) Stills, movie of Moesin-GFP-expressing embryo. Times in hours:minutes. (I) Before denticle formation. (J,K) Actin accumulates apically in presumptive denticle belts (brackets). (L) Actin foci along the posterior margin (arrows). (M-O) Foci condense and brighten. White arrows, red arrowheads – foci merging. (P) Denticles begin elongation. Scale bars, 10 µm.





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