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Figure 3


Fig. 3. MT and P-Tyr localization during denticle development. (A-D) Embryos, anterior left (A,D) or upper left (B,C). F-actin (phalloidin, green), P-Tyr (A,B red) or MTs (C,D red). (A) P-Tyr (red) accumulates with and around actin (green) in condensations (arrows). (B) P-Tyr (red) is enriched at denticle base (white arrow) but less prominent at the tip (blue arrow). (C) MTs (red) form circumferential arrays without enrichment near condensations (green, arrows). (D) MTs enriched at the denticle base (blue arrows) and in the core (white arrows) during elongation. (E,F) Deconvolved confocal images. (E) F-Actin (phalloidin). (E1) Early condensations. (E2) Late condensations with `donut holes'. (E3,E4) U- or V-shaped denticles. (F) MTs (red). F-actin (Phalloidin; green). (F1) MTs encircling a condensation. (F2,F3) MTs in the core of V-shaped denticles. (F4) Fully elongated denticle. MTs at base and in core. Scale bars, 5 µm.





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