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Fig. 4. Dia in denticles. Embryos, anterior to top. Dia (red), F-actin (phalloidin; green). (A) Before denticle development. (B) As denticles elongate, Dia becomes enriched in denticles (white arrowheads) while remaining cortical (blue arrowheads) in non-denticle-producing cells. (C-E) Deconvolved confocal images. (C) No substantial enrichment in early actin condensations (arrowhead). (D) Dia begins accumulating in elongating denticles (arrowheads). (E) Dia accumulates in a punctate fashion in elongated denticles (arrowheads). Scale bars, 5 µm.