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Fig. 5. Arp3 and Ena in denticles. Embryos, anterior to top. (A-C) Deconvolved confocal images, Arp3 (red), F-actin (phalloidin; green). (A) Arp3 puncta within and surrounding actin condensations (arrowhead). (B) Arp3 enrichment as denticles elongate (arrowheads). (C) Arp3 in elongated denticles (arrowhead). (D-I) Ena (red or single channel), F-actin (phalloidin; green). (D) Denticle-producing cells accumulate cortical Ena (brackets); in other cells Ena remains at tricellular junctions (arrowheads). (E,F) Ena enriched in elongating denticles (brackets). Arrowheads, tricellular junctions in cells that do not make denticles. (G-I) Deconvolved confocal images. Blue arrowheads Ena in tricellular junctions. (G) Actin condensations without Ena enrichment (yellow arrowhead). (H) Ena in elongating denticles (yellow arrowhead). (I) Ena in elongated denticles (yellow arrowhead). Inset, elongated denticle in cross section Ena in denticle and at its base (white arrowhead). Scale bars, 5 µm.